Abstract: A duck hepatitis A virus 1 (DHAV-1) , designated pancreatitis-type DHAV-1, has been isolated from Muscovy ducklings with yellow or hemorrhagic pancreas.To elucidate different pathogenicity of DHAV-1infection between pancreatitis-type and classical ducklings, Muscovy duck, mallard duck, Cherry valley duck and Maya duck, raised for 7, 14, 21, 28and 35drespectively, were inoculated with pancreatitis-type and classical DHAV-1.The results showed that Muscovy duck was the most sensitive to pancreatitis-type DHAV-1, followed by mallard duck and Cherry valley duck, and the Maya duck was the lowest.However, Cherry valley duck was the most sensitive to classical DHAV-1, followed by Muscovy ducks.Hemorrhagic and yellow pancreas was detected in Muscovy duckling and mallard duckling infected with pancreatitis-type DHAV-1, but no hemorrhage in liver.The above indicated that there were obviously different pathogenicity between pancreatitis-type and classical DHAV-1in sensitive host, and gross lesions.