Abstract: Tianyou 3301was developed by crossing the good quality three-line male sterile“Tianfeng A”with the restorer line“Minhui 3301”.The cultivated area of Tianyou 3301is 300 000hm2from 2010to 2012, the total yields were about 150million kg in three years.It is characteristic of good plant types, super-high yield, good quality, blast resistance, wide adaptability, large panicles and more spikelets, high setting rate, strong ratoon ability and high temperature tolerance at heading stage.It was registered in Fujian province and the National Crops Variety Approval Committee in 2008and 2010, respectively, and was approved in Hainan province in 2011.It was one of those super-hybrid varieties to be demonstrated and extended in national wide.The extension and application, botanical traits and yield components of Tianyou 3301were summarized in this paper.And its yield, resistance and quality in regional trials, producing trials and high yield demonstration were introduced in the paper.