Abstract: The effects of five sowing quantity(135,180,225,270,315kg·hm-2)on barley seedling Grain Yield and quality were studied.The results showed that barley seedling yield and quality were significant differences among different sowing amount treatments.Appropriate increasing seeding rate was conducive to improve the yield of barley seedling.But when the sowing quantity was over 225kg·hm-2,increasing seeding rate could not improve barley seedling′s yield.At the same time,with increasing seeding rate,the chlorophyll and soluble protein content decreased and SOD activities increased at first and then decreased.The test showed that the suitable sowing amount for barley seedling production was about 225kg·hm-2.