无患子组织培养与快速繁殖技术研究

Research on Tissue Culture and Fast Propagation Technology of Sapindus mukurossi

摘要: 以无患子生长健壮的幼嫩枝条为材料,研究其组培快繁技术。结果表明:①芽体启动培养的最适宜培养基为MS+NAA 0.2mg·L-1+IBA 0.05mg·L-1+6-BA 3.0mg·L-1,萌芽率最高,达94.01%;②增殖培养的最佳培养基组合为MS+IBA 0.02mg·L-1+6-BA 1.0mg·L-1,增殖系数最高,为4.20;③在生根培养中,1/2MS+IBA 0.5mg·L-1+NAA 0.1mg·L-1最适宜诱导生根,生根率高达80.47%。炼苗移栽后成活率高达89.21%。

Abstract: Vigorous young stem segment of Sapindus mukurossi were used as explants for tissue culture and fast propagation.The results showed that the optimum medium formula of bud induction culture was MS + NAA 0.2 mg·L-1+IBA 0.05mg·L-1+6-BA 3.0mg·L-1and the bud rate reached 94.01%.The optimum combination of multiplication culture was MS+IBA 0.02mg·L-1+6-BA 1.0mg·L-1.During rooting culture,1/2MS+ IBA 0.5mg·L-1+ NAA 0.1mg·L-1 was the optimum medium.The highest rooting rate is 80.47%.After refine seeding transplant,the survival rate was maximal for 89.21%.