番茄青枯病植物疫苗胶悬剂保存特性的研究
Study on Storage Characteristics of Colloidal Suspension Agent Used us Plant Vaccine Against Tomato Bacterial wilt Disease
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摘要: 采用50L全自动发酵罐进行植物疫苗生产菌株FJAT-1458发酵液制备,将其制成琼脂终浓度为2‰的胶悬剂,研究pH、氯化钠浓度对植物疫苗胶悬剂保存特性的影响,并跟踪检测植物疫苗胶悬剂保存过程致病性及防效的变化。结果表明,植物疫苗胶悬剂保存的最适盐浓度为2%~3%,最适pH值为6~8。植物疫苗胶悬剂保存过程中,菌株对番茄青枯病的防效稳定不变,菌体的致病性指标-弱化指数变化小,从生物学特性可视为变化无差异。Abstract: The fermentation of Ralstonia solanacearum FJAT-1458 used for production of plant vaccine was conducted in 50Lautomatic fermentation container,and the fermentation broth was prepared to produce with 2‰ agar to form a plant vaccine.During plant vaccine colloidal suspension agent storage process,the characteristics of strain FJAT-1458was detected under different pH values and different percentages of salt,and the control efficiency and attenuation index of strain FJAT-1458were also detected.The results showed that the best storage condition for plant vaccine colloidal suspension agent was pH 6-8and 2%-3% of salt.The Control efficiency of plant vaccine colloidal suspension agent against tomato bacteria wilt during its storage was stable and there was no significant difference between the attenuation in biological view.