Abstract: Monascus purpureus S was used to research the effects of different vitamins on pigment production and color hue of fermentation liquor.The result indicated that niacin and folic acid were obvious promoted pigment production,the color value was up to about 80U·g-1.The color tone value of Monascus pigment production was 1.40when adding 0.03% niacin,which would lead to obtain high color value with red tone.Based on vitamin single-factor experiment,the culture medium of Monascus was optimized by quadratic general rotary design and a quadratic regression equation was established.The optimum culture medium of Monascus was obtained by variance analysis and the optimization of mathematical model,which were 3.97% rice powder,3.37% monosodium glutamate,0.024% Mn2+and 0.04% vitamin PP.Under those conditions,the highest color value reached with estimated value and cerified value being of 107.3U·g-1 and 106.7U·g-1.