Abstract: A latex agglutinin test for detection of duck Tembusu virus has been developed by sensitizing latex with monoclonal antibody of duck Tembusu virus WR strain.Under the optimum sensitization of latex particles with 300μg monoclonal antibody at 37℃for 3h,the latex agglutination test could reach a detection limit of 10-2.5 ELD50duck Tembusu virus.A total of 73samples collected from field cases were detected by latex agglutination test(27positive)and RT-PCR(31positive),indicating the coincidence rate of the two methods was 87.1%in practical utilization.The results showed that the latex agglutinin test for detection of duck Tembusu virus was facile,rapid and visual,which could be available for clinical utilization.