Abstract: Mango seedlings were used as the test material in this experimentation to optimize SRAP by the single factor tests of primer DNA,primer,and 2xTaq PCR Master Mix.The results indicated that a 20uL reaction system appeared to be most desirable,as the resultant electrophoresis provided clear amplification bands and was highly polymorphic while the procedure was simple.From 81primer combinations,29were selected with abundant polymorphism.The optimized SRAP-PCR system and polymorphism primer combinations could be applied for molecular genetic studies on mango.Our investigation showed that annealing temperature was not a significant factor on the amplification.