胰腺炎型鸭1型甲肝病毒结构蛋白VP1基因的克隆和表达

Cloning and Prokaryotic Expression of the VP1 Gene of Pancreotropic Duck Hepatitis Type 1 Virus

摘要: 通过RT-PCR方法扩增出MPZJ1206株胰腺炎型鸭1型甲肝病毒结构蛋白VP1基因, 将其与原核表达载体pGEX-6P-1连接获得重组表达质粒pGEX-VP1, 进行条件优化诱导表达, 将表达的重组蛋白经SDS-PAGE分析。结果表明, 分子量约为52kDa的重组目的蛋白得到表达。该研究为开发胰腺炎型鸭1型甲肝病毒诊断方法和研究VP1蛋白功能奠定基础。

Abstract: The structure protein (VP1) gene of pancreotropic duck hepatitis type 1virus MPZJ1206 strain was amplified by RT-PCR and integrated into the prokaryotic expression vector pGEX-6P-1, then expressed in E.coli.The SDS-PAGE results of the expressed protein demonstrated that the expression of recombinant VP1 protein of 52 kDa was in a high level, which laid a good foundation for the molecular diagnosis development and the function research of the VP1 protein.