Abstract: The chitinase activity of Paenibacillus chitinolyticus FJAT-10038 was characterized by filtering FJAT-10038 Bacillus using chitinase medium, the chitinase activity of which reached at 11.044 U mL-1.Furthermore, Acetone extracts of FJAT-10038 Bacillus were analyzed by GC-MS.Our results suggest that thirteen-one compounds are identified, and the content of Pyrrolo 1, 2-apyrazine-1, 4-dione, hexahydro-and Propane, 1-ethoxy-2-methyl-reached 44.1672%and 15.5814%.Meanwhile, the match degree of Butylated Hydroxytoluene, Pyrrolo 1, 2-apyrazine-1, 4-dione, hexahydro-and Pyrrolo 1, 2-apyrazine-1, 4-dione, hexahydro-3- (phenylmethyl) -were 90%;97% and 93%, with a relative amount by 0.4305%, 44.1672% and 0.3842%.In addition, some other active substances were also been characterized.