Abstract: Tobacco cultivars, Yunyan 97, Yunyan 99, Honghuadajinyuan, Zhongyan 103, Yunyan 100, NC297, NC71, Xingyan No.1, K326 and Yunyan 87 (ck) , were used to evaluate the effect of fertilization on the agronomic characteristics, botanical properites, disease resistance, visual quality and economic performance of the tobaccos grown in Ankang.The results showed that the agronomic characteristics, plant height, stem girth and lumbar leaf length of the tested cultivars increased with increased fertilization.But, the increases slowed down as the amount of the fertilizer was further increased.As for the overall disease resistance, Yunyan 97, Yunyan 99, Yunyan 100 and NC297were better among all tested varietis.Economically, the yield and output of Yunyan 97, Yunyan 100, NC297 and Yunyan 99 performed well when the intermediate fertilization was applied.