Abstract: To analyze the pathogenicity, medicine sensitive, serotype and virulence associated genes of Escherichia coli (E.coli) strains from salpingitis ducks, the livers of ducks typically showing salpingitis were collected, from which the E.coli was isolated and identified.Pathogenicity of E.coli isolates were determined by subcutaneous injection of susceptible ducks.Serotype of E.coli isolates were detected by agglutination test.Virulence associated genes were detected by PCR.16 strains of duck E.coli were identified, among which there were 11 virulent strains.More than 70%of the strains were sensitive to Amikacin, Florfenicol, Cefazolin, Levofloxacin and Spectinomycin.More than 70% of the strains were resistant to Trimethoprim sulfamethoxazole, Kanamycin, Gentamicin, Enrofloxacin and Ciprofloxacin.These strains were belonged to 4serotypes, among which O78 was the main serotype which accounted for 56.3%.The detection rate of irp2 gene, fyuAgene, fimCgene and papCgene were68.8%, 87.5%, 75%, 100%.16 strains of E.coli were isolated from salpingitis ducks and O78 was the main serotype.Spectinomycin and Florfenicol were the suitable drugs.