Abstract: Based on the previously obtained overexpression transgenic plants with the ubiquitin ligase, OsHUB2, from rice, expression lever of the enzyme was studied.Three pairs of primers, OsHUB2-F1R1, OsHUB2-F2R2 and OsHUB2-F3R3, were designed using the Primer Premier 5.0according to the sequence of the enzyme from GeneBank.Subsequently, RT-PCR was conducted to select the best primer pairs with a specific and single amplification product.The results showed OsHUB2-F3R3 to be desirable on both and functions.The analytical procedure was thus considered of use in future studies on the rice ubiquitin ligase.