Abstract: To study the effect of Biogas slurry application on seed germination and physiological indices of sand culture ryegrass, ryegrass seeds were treated by four different slurry application rates, and then the seed morphological and seedling physiology indices were analyzed.Resultsshowed that treatments have no effect on the germination of sand culture ryegrass.Ryegrass seed germination rate and power were increased first and then decreased with the increase of irrigation biogas slurry application rates.The treatment of T1 biogas slurry application rate with the highest germination rate and germination power which significantly higher than the treatments of T3 and T4biogas slurry application rates (P<0.05.Biogas slurry application could increase the shoot height and the root length of Ryegrass seedling, and the treatment of T1 biogas slurry application rate with the best effect.The chlorophyll content and root activity were increased first and then decreased, and both the peak value of them were the treatments of T1 biogas slurry application rate.The POD and MDA had no significant differences among treatments (P>0.05) .SOD and CAT increased with the increasing of irrigation biogas slurry, but with differences on the rate and trend of increase.Our results suggest that ryegrass seeds could germinate in the sand and the treatment of T1 biogas slurry application rate has the optimum benefit for it.