Effects of Biogas Slurry Application on Seed Germination and Physiological Effects of Sand Culture Ryegrass
-
摘要: 为研究沼液灌溉对砂培黑麦草种子发芽及生理效应的影响, 设置4个梯度沼液灌溉量分别灌溉砂培黑麦草, 分析种子发芽情况和幼苗生理学指标的变化。结果表明:各处理均能正常发芽, 黑麦草种子的发芽率和发芽势随着灌溉沼液量的增加呈现先上升后下降的趋势, 其中以T1试验组的发芽率和发芽势最高, 显著高于T3、T4试验组 (P<0.05) ;沼液灌溉能促进黑麦草幼苗苗高和根长, 试验组T1的促进效果最好;各试验组叶绿素含量和根系活力均随着沼液灌溉量的增加先上升后下降, 且二者的峰值均为T1试验组;不同稀释比沼液试验组间黑麦草幼苗POD酶活性、MDA含量差异不显著 (P>0.05) , SOD、CAT酶活性随沼液灌溉量的增加而增加, 但增加的幅度和趋势存在差异。综合分析认为砂培模式下黑麦草种子能很好地发芽并快速生长成幼苗, 且最适灌溉的沼液与蒸馏水的稀释比为1∶3。Abstract: To study the effect of Biogas slurry application on seed germination and physiological indices of sand culture ryegrass, ryegrass seeds were treated by four different slurry application rates, and then the seed morphological and seedling physiology indices were analyzed.Resultsshowed that treatments have no effect on the germination of sand culture ryegrass.Ryegrass seed germination rate and power were increased first and then decreased with the increase of irrigation biogas slurry application rates.The treatment of T1 biogas slurry application rate with the highest germination rate and germination power which significantly higher than the treatments of T3 and T4biogas slurry application rates (P<0.05.Biogas slurry application could increase the shoot height and the root length of Ryegrass seedling, and the treatment of T1 biogas slurry application rate with the best effect.The chlorophyll content and root activity were increased first and then decreased, and both the peak value of them were the treatments of T1 biogas slurry application rate.The POD and MDA had no significant differences among treatments (P>0.05) .SOD and CAT increased with the increasing of irrigation biogas slurry, but with differences on the rate and trend of increase.Our results suggest that ryegrass seeds could germinate in the sand and the treatment of T1 biogas slurry application rate has the optimum benefit for it.
-
Keywords:
- biogas slurry /
- ryegrass /
- sand culture /
- germination
-
-
陈志刚, 张红蕊, 周晓红, 等.铝胁迫对黑麦草种子萌发和幼苗生长的影响[J].水土保持研究, 2011, 18 (4) :207-210. 任勇翔, 杨春辉, 孙军峰, 等.冬小麦和黑麦草作为寒冷季节人工湿地栽培作物处理城市污水效果研究.[J]西安建筑科技大学学报:自然科学版, 2012, 44 (5) :714-719 、735.
徐晓锋, 杨林章, 许海, 等.黑麦草水培系统对化粪池粪污滤液中氮磷净化效果[J].应用生态学报, 2006, 17 (10) :1815-1819. 陈钟佃, 唐龙飞, 黄秀声, 等.沼液对2种禾本科牧草生产性能的影响[J].江西农业学报, 2007, 19 (12) :42-44. 黄勤楼, 钟珍梅, 陈恩, 等.施氮水平与方式对黑麦草生物学特性和硝酸盐含量的影响[J].草业学报, 2010, 19 (1) :103-112. 高承芳, 刘远, 张晓佩, 等.多花黑麦草品种在福建地区的引种表现[J].福建农业学报, 2012, 27 (4) :417-421. 黄秀声, 黄勤楼, 杨信, 等.浇施沼液对狼尾草植株硝酸盐累积及其氮素利用效率研究.[J]草业学报, 2012, 21 (3) :61-68. 唐华, 郭彦军, 李智燕.沼液灌溉对黑麦草生长及土壤性质的影响[J].草业学报, 2011, 19 (6) :939-942. 王宗寿.利用沼液种植黑麦草对土壤环境质量的影响[J].农业环境科学学报, 2007, 26 (S) :172-175. 吴冬青.沼液营养液在蔬菜无土栽培上的应用研究[D].保定:河北农业大学, 2012. 陈敏娇.畜禽养殖污水植物净化与资源化利用研究[D].杭州:浙江大学, 2007. 吴翠云, 陈佳, 阿依买木, 等.低温与激素处理对解除杜梨种子休眠的研究[J].北方园艺, 2009, (4) :47-49. XUE Y F, LIU L, LIU Z P, et al.Protective role of Ca against NaCl toxicity in Jerusalem artichoke by up-regulation of antioxidant enzymes[J].Pedosphere, 2008, 18 (6) :766-774.
王学奎, 植物生理生化实验原理和技术[M].第2版.北京:中国高等教育出版社, 2006:118-119, 167-173 , 190-192, 280-281.
扈学文, 许秋瑾, 金相灿, 等.不同分子量壳寡糖对黑麦草种子萌发和幼苗抗病酶活性影响的研究[J].中国农学通报, 2007, 23 (2) :221-225. 张秋月.沼液在作物侵种和催芽中的应用[J].河北农业科技, 2008, (3) :40. 薛延丰, 石志琦, 严少华, 等.利用生理生化参数评价水葫芦沼液浸种可行性初步研究.[J]草业学报, 2010, 19 (5) :51-56. 蔡艳蓉, 高照良, 赵晶, 等.不同基质与施肥水平对黑麦草叶绿素及根系生长情况的影响[J].水土保持研究, 2011, 18 (4) :157-159 , 165.
甘露, 范海燕, 吴文勇, 等.无土栽培基质水分特性参数研究[J].农业机械学报, 2013, 44 (5) :113-118 , 142.
潘颖, 李孝良.近红外光谱技术定量测定基质参数研究[J].安徽农学通报, 2007, 13 (5) :55-56. 薛延丰, 李慧明, 石志琦.微囊藻毒素 (MC-RR) 对白三叶种子萌发及幼苗生理生化特性影响[J].草业学报, 2009, 18 (6) :180-185. 钟珍梅, 黄秀声, 黄勤楼.水葫芦净化沼液效果的研究[J].福建农业科技, 2006, (2) :84-86.
计量
- 文章访问数: 204
- HTML全文浏览量: 51
- PDF下载量: 2
下载:
