Abstract: The envelop protein fragments of avian Tembusu virus were amplified by RT-PCR from the identified avian Tembusu virus isolated in Fujian, and cloned to the T-vector for sequenceing and bioinformatics analysis.The results revealed that the avian Tembusu virus envelop gene contained 1 053 nucleotides, coding an open reading frame (ORF) with 501 amino acids.The molecular weights, theoretical isoelectric point and the instability index of avian Tembusu virus envelop protein were 54.217 ku, 6.89 and 25.48, respectively.The topology predicted by Nbest using the TMHMM2.0is o442-464i471-489 o.The sequenced envelop protein shared the highest homogeneity with Peking duck-origin avian Tembusu virus strain (GenBank accession number is JF459991) , which was 99.8%, while shared with common sheldduck-origin avian Tembusu virus strain (GenBank accession number is JQ928189) 98.2% homogeneity.The results demonstrated that avian Tembusu virus had no host specificity