Abstract: Nutrient constituents of the litter of feeding pigs in the microbial fermentation bed were determined and effects on the mycelial growth of five edible fungi in different formulas added with litter of different proportions were studied.The results showed the content of nitrogen was 1.63% in litter providing good nitrogen source for the mycelial growth of edible fungi, mycelium of five edible fungi could grow in different formulas, suitable proportion of litter in the substrate can promote mycelial growth, the highest mycelial growth rate of Pleurotus ostreatus was9.50 mm d-1 when the formula contained 35% litter, the highest mycelial growth rate of Auricularia polytricha was 4.50 mm·d-1 when the formula contained 40% litter, the highest mycelial growth rate of Flammulina velutipes was 4.35mm·d-1 when the formula contained 35%litter, the highest mycelial growth rate of Pholiota nameko was 4.52mm·d-1 when the formula contained 20%litter, the highest mycelial growth rate of Hypsizigus marmoreus was 2.60mm·d-1 when the formula contained 45%litter, they were very significantly higher than the control (P<0.01) .