Abstract: With no tissue culture chewing cane seedling was used as control, 4kinds of endogenous hormones of the somaclone offspring were tested, and the mechanism of tillering of chewing cane and control technology was studied by exogenous hormone treatment.Resultsshowed that IAA and CTK content of somaclone offspring were higher than control, which suggested that they might play a role in the process of tillering, but ABA and GA3 content was not significantly different from control.The result of exogenous hormone treatment showed that 6-BA treatment could significantly improve tillering rate of chewing cane, and NAA had the certain effect on the inhibition of chewing cane tillering.Comprehensive the result above, we speculated that the effect factors of chewing cane somaclone tillering might be the 6-BA which was remained in the process of tissue culture.