Abstract: Long-term field experiment is an important way to study long-term ecological process with its environmental effect and control measure of cropland.Fertilization and cultivation in the agricultural production are the two most common and frequent management practices, which have a profound impact on agricultural productivity and ecological processes.Publications were collected under long-term agro-ecosystem experiments with different fertilizations and conservation tillage treatments which were availabled from the Chinese Journals Database (CNKI) and the Chinese Journals of Science and Technology Database (VIP Info) during 1979 to 2009.And informations of trial site distribution, soil types, crop systems, monitoring durations and site set-up time, management measures, research content, testing area and oriented units were extracted from the publications above to analyze the long-term experiment status and distribution characteristics, and discuss the existing problems, progresses and proposed countermeasures of long-term agro-ecosystem experiments in mainland China.