Abstract: Plantlets from the elite, high essential oil containing Melaleuca altennifoliatended to turn brown and die after generations of multiplication.In addition, the low rooting rate prohibited the commercialization of the tissue cultivation.Using Hyponex No.1, B5 and MS as the basis, media formulated with varied concentrations of substrates, vitamins and growth regulators were used in an attempt to improve the cultivation.The results showed that (a) 3.0g·L-1 of Hyponex No.1in combination with VH, Vc and VB2 effectively inhibited the browning and death of the plantlets; (b) a supplementation of 0.4-0.6 mg·L-1 GA in the subculture medium increased the number of effective seedlings, and encouraged a strong and uniform growth of the seedlings;and, (c) the addition of Hyponex No.1at a rate of 2.0g·L-1 in the rooting medium raised the average rooting rate for different individuals to 95%.