羊传染性脓疱病毒福州株的分离和初步鉴定

Isolation and Iidentification of a Fuzhou Strain of the Orf Virus

摘要: 从福建省福州地区养羊场发病羊组织中分离到1株羊传染性脓疱病毒ORFV-FZ株。对ORFV-FZ株B2L基因片段进行扩增和序列分析,结果显示,ORFV-FZ株与国内福建、新疆、广西等毒株的核苷酸同源性在99%以上,其中与FJ-YX株的同源性为99.8%,表明ORFV-FZ株可能与FJ-YX株有着共同的来源。

Abstract: In this study,an Orf virus strain,named ORFV-FZ,was isolated from the infected goat in the farm of Fuzhou in Fujian.The B2 Lgene fragment of the ORFV-FZ strain was amplified and sequenced.The sequencing result showed that the nucleotide homology of the ORFV-FZ strain with those strains from Fujian,Xinjiang and Guangxi were above 99%,in which,the homology between ORFV-FZ and FJ-YX strain was up to 99.8%,indicated the possibility of the same origin of these two strains.