Abstract: For medicine selection and safety assessment of disease control of Pomoxisnigromacufastus,the acute toxicity test of seventeen aquaculture drugs were done for Juvenile Pomoxis nigromacufastus.The results showed that the safety concentrations of seventeen aquaculture drugs from the highest to the lowest were natrii chloridum(220mg·L-1),mequindox(16.75 mg·L-1),baichongke(10.72 mg·L-1),meijunjing(7.98 mg·L-1),florfeniol(7.52mg·L-1),mebendazolum(3.44 mg·L-1),liquor formaldehyde(3.16 mg·L-1),polyiodide(1.94mg·L-1),kalii permanganas(1.07mg·L-1),yuchongkexing(0.76mg·L-1),xiaoguazhihuanjing(0.39mg·L-1),trichlophonus(0.35 mg·L-1),baitechongqing(0.32 mg·L-1),xinyiwei(0.3 mg· L-1),trichlorinafed isocyanuric acid(0.06 mg·L-1),baiteyichongdi(0.031 mg·L-1),and cupric sulfate(0.028mg·L-1).The safety concentrations of cupric sulfat for Juvenile Pomoxis nigromacufastus were significantly lower than commonly used concentrations.Cupric sulfate cannot be used to cure the disease of Juvenile Pomoxis nigromacufastus. The safety concentrations of Trichlorinafed isocyanuric acid,Liqour formaldehyde, Kalii permanganas,Yuchongkexing and Natrii chloridum for Juvenile Pomoxis nigromacufastus were lower than their commonly used concentrations,so they should be used carefully.The safety concentrations of eleven kinds of drugs of mebendazolum, polyiodide, meijunjing, baichongke, xiaoguazhihuanjing, xinyiwei, baitechongqing,trichlophonus, mequindox,florfeniol and baiteyichongdi were near or higher than their commonly used concentrations,so they could be used safely in cultivation of Pomoxis nigromacufastus.