37份药用石斛种质资源亲缘关系的ISSR分析

Relationships Among 37 Germplasm Resources of Medicinal Dendrobium Based on ISSR

摘要: 利用ISSR技术对37份药用石斛属种质资源进行亲缘关系分析,从备选72条引物中筛选出15条引物,共扩增出89条谱带,有89条多态带,多态性条带比例为100%。采用NTSYS-PC2.0软件分析结果,37份石斛种质资源遗传相似系数在0.50~0.85。聚类结果显示,以相似系数为0.55,可将37份石斛种质明显划分为7个类群,表明药用石斛植物遗传多样性丰富,ISSR技术可应用于药用石斛的亲缘关系研究。

Abstract: Genetic relationship of 37 Dendrobium was studied by ISSR technique.Fifteen primers with clear and polymorphic bands were screened out from 72 ISSR random primers.Eighty-nine locus were detected with polymorphic percentage in 100%.As analyzed by NTSYS-PC2.0,the genetic similarity of 37 Dendrobium were ranged from 0.50-0.85 and grouped into 7sub-cluster when the similarity coefficient was 0.55.The results indicated that plants of medicinal Dendrobium were rich in genetic diversity and their genetic relationship could be studied by ISSR technique.