Abstract: To investigate acute toxicity and antioxidant capacity,seleniferous Pleurotus geesteranus mycelia(SP)were given to groups of ICR mice and D-galactose induced oxidative damaged mice,respectively.The maximal tolerance dose(MTD)in SP for mice was greater than 20.0g·kg-1 body weight,which defined SP no risk in acute toxicity.Comparing with model control group(CK-M),the MDA and protein carbonyl in mouse serum were decreased significantly by 20.08%,22.19%,39.26% and 38.31%,44.16%,25.32% for SP-L,SP-M,SP-H groups,respectively;while GSH-Px of mice in three SP groups was increased(P<0.05),especially in SP-M and SP-H groups for 18.5%.However,there were no significant differences between three SP groups with CK group in serum MDA,protein carbonyl and GSH-Px contents.The results indicated that the protective effect of SP might enable in restoration for oxidative damaged mice getting back to normal like those in control group.