Abstract: Cultivation characteristic of seven Pleurotus geesteranus strains were comparatively studied,their genetic diversity were analyzed by identifying molecular marker of ISSR.The results showed that strains P817 and P618were excellent in comprehensive agronomic traits and the genetic similarity coefficient for tested strains ranged from0.29 to 0.73.Seven strains were divided into 4groups when genetic distance was at 0.59.The strains P817 and P618were grouped differently by genetic distance.The results provide theoretical reference in genetic assessment for Pleurotus geesteranus.