斑茅纤维液态酶解糖化工艺优化研究

Saccharification of Erianthus arundinaceum Fibers by Liquid Enzymatic Digestion

摘要: 采用斑茅纤维为原料,用2%NaOH和1%H2O2进行预处理,探讨纤维素酶液态发酵法糖化工艺的优化,分析糖化材料、时间、pH、纤维用量、温度和转速等单因素对纤维素产糖的影响。结果表明:糖化材料用2%NaOH和1%H2O2处理,反应时间在28~30h,pH在4.5~5.5,纤维用量在4~6g,温度在50~55℃,转速约为180r·min-1时斑茅纤维水解的效果最好。通过正交试验设计确定最佳糖化条件:糖化温度52℃、时间30h、纤维用量5g、pH 6.0,糖化率达到45.21%。

Abstract: Liquid digestion of Erianthus arundinaceum fibers in the solution containing 2%NaOH and 1%H2O2to achieve the desired cellulase saccharification was studied.Effect of the substrates,digestion time,pH,fiber content,temperature and agitation speed on the process were analyzed.The conditions studied for the fiber hydrolysis included the applications of 2% NaOH and 1%H2O2,28 to 30hof reaction time,pH4.5to 5.5,4to 6gof fiber,temperatures between 50℃ and 55℃,and agitation speeds of approximately 180r· min-1.The orthogonal experiment indicated the optimal conditions to reach a saccharification rate of 45.21% were 30 h,pH6.0,5g fiber and 52℃.