铁皮石斛白绢病菌的分离鉴定与抑菌药剂筛选

Isolation of Pathogen and Fungicide Selection for Sclerotial Blight in Dendrobium officinale

摘要: 通过对分离到的铁皮石斛白绢病菌进行形态特征观察、致病性测定及rDNA-ITS的同源性分析,确定侵染铁皮石斛的病原菌为Sclerotium rolfsii Sacc.(有性态为Athelia rolfsii),与罗氏阿太菌(Athelia rolfsii,登录号:HQ420816.1)同源性达到100%。选择5种药剂多利维生、80%乙蒜素、三唑酮、百菌清、奥力克-霉止,采用菌丝生长速率法,对白绢病菌进行室内抑菌测定。经室内抑菌试验及方差分析结果表明,多利维生与80%乙蒜素抑制率均可达87%以上,与对照相比达极显著水平。

Abstract: The pathogen that causedSclerotialblight in Dendrobium officinaleKimura et Migo was identified to beSclerotiumrolfsii Sacc.by its morphological characteristics,as well as through the pathogenicity test and sequence homology analysis of its rDNA-ITS.The ITS sequence of the funguswas 100% identical with that of Atheliarolfsii(GenBank ID:HQ420816.1).Subsequently,a toxicity test on 5fungicides for controlling S.rolfsii was conducted in the laboratory by using mycelium growth rate method and variance analysis.The results showed thatpolyversum and 80% ethylicin hada significant effect on controlling the pathogen with aninhibitory rate exceeding 87%,which reached an extremely significant level.