Abstract: Seeds of Cymbidium ensifolium var.susin were used as the explant tissue to study the aseptic sowing and rapid propagation in situcultivation for the plant.Effects of the medium(MS,MS,Hyponex No.1or improved MS)and phytohormone(6-BA,TDZ,and/or NAA)on the mass breeding during seed germination,rhizome multiplication,and rhizome differentiation stages.The results showed that the 1/2MS medium with 6-BA 3.0mg·L^-1,NAA 0.5mg·L^-1,LH 2.0g·L^-1,AC 1.0g·L^-1 and sugar 30g·L^-1 was conducive to the seed germination;the improved medium No.1with TDZ 1.0mg·L^-1,NAA 0.5mg·L^-1,AC 0.5g·L^-1 and sugar30g·L^-1 was favorable for the rhizome multiplication(the propagation coefficient reached 8.1in 45days);and,the improved medium No.1with 6-BA2.0mg·L^-1,NAA 0.1mg·L^-1and sugar 30g·L^-1for 45 days followed by transferring the subculture onto the MS with IBA 0.5mg·L^-1,AC 0.5g·L^-1and sugar 20g·L^-1for 60 days was bestfor rhizome differentiation.At the end,young plantlets emerged and developed into mature plants with a rhizome differentiation rate of 86.3% and a rooting rate of 100.0%.