Abstract: Extracellular metabolites of Brevibacillus choshinensis FJAT-10008 were analyzed by liquid chromatographyhybrid quadrupole time-of-flight mass spectrometry and Nigericin from FJAT-10008 was tested. Molecular Feature Extraction(MFE)and database retrieval were applied to access the information of overall metabolites of B.choshinensis.As a result,1246 compounds were detected and 206 of them were identified.The match score of Nigericin was 92.12 and its relative content was 1.45%.The retention time of Nigericin was about 28.7017 min,and its accurate mass was724.4679.The results provided a theory basis for development and utilization of polyether antibiotics fromB.choshinensis.