Abstract: In order to further study the utilization of spent litters,the greenhouse gas emission during the composition of spent litters in deep-litter system were determined in this paper,and the effects of microbial agents were studied.The results showed that the spent litters in deep-litter system could compost successfully whether using microbial agents or not.But the microbial agents could shorten composting time.The CO2 and CH4emission of spent litters treated by microbial agents were higher than the control,whereas the N2 O emission of high temperature was obviously lower than the others.The total greenhouse gas emission could be reduced by 9.1%treated by FC-1microbial agents compared with the control.