Abstract: Protein targeting is an important tool to determine the functionality of a protein. Adual targeted protein isa protein that located in two subcellular locations. It was estimated that more than 400 proteins were putatively targeted to mitochondria as well as plastid based on the ambiguous N-terminal pre-sequences in land plant genomes. In this study, the dual targeted proteins were searched against the protein databases of Uniprot, Mito P2, MGI, TAIR, and DBMLoc as well as the existing publications. A total of 703 proteins localized in both mitochondria and plastid in plants were collected. Whilst, 829 exclusive mitochondrial and 6 376 exclusive plastid proteins were downloaded from Uniprotfor comparison. The net electric charges of the dual targeted proteins were analyzed based on these 3 data sets. The results indicated the net charge of the plant dual targeted proteins was significantly lower than that of the proteins exclusively existed in mitochondrial or plastid. The charge differentiation provideda critical clue for the study of the molecular mechanism of dual targeted proteins in plants.