Abstract: In this experiment, HOS1 promoter was cloned successfully from the 'Tianbao' banana, and was further bioinformatically predicted. The analysis results showed that the sequence similarity of HOS1 promoter was 92.43% between the Tianbao banana and the wild banana in Malaysia, and the Tianbao banana promoter contained 35 kinds of cis-elements, including a variety of types of phytohormone response cis-elements and cis-elements concerning abiotic stress, which suggested that there be a close relationship with cold stress response in Tianbao banana. Tianbao banana HOS1 promoter contained two CpG islands, which suggested it should also have a close relationship between cold stress response and methylation in Tianbao banana.