Abstract: Extracellular metabolites of Bacillus aryabhattai FJAT-14220 during fermentation were analyzed with liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry. Saquinavir in the fermentation broth was targeted for the study. Molecular feature extraction and database retrieval were applied to access the information on the metabolites of B. aryabhattai. A total of 883 compounds were detected with 148 of them identified. The matching score of saquinavir was 93.08 with a relative concentration of 1.73%. Its retention time was 2.7191 min; and, accurate mass, 670.3837. The results could be the foundation for future development and utilization of saquinavir by B. aryabhattai fermentation.