Abstract: The σC gene of muscovy duck reovirus (MDRV) was amplified by RT-PCR and cloned into pShuttle-CMV plasmid after the amplicon was digested by XhoI+Hind III enzyme. Sequencing analysis confirmed the gene to be σC. Subsequently, the recombinant plasmid pShuttle-CMV-σC and the adenovirus backbone vector, pAdEasy-1, were co-transfected into 293 cells to construct a recombinant adenovirus. The obtained recombinant adenovirus pAd-σC was positively identified by PCR and RT-PCR. The information obtained could be used for genetic engineering a vaccine against MDRV.