Abstract: Most phenotypic functions come from the interaction between genes and proteins. Protein-protein interaction (PPI) is an important technology that has developed rapidly in recent years, to study protein function and this technology plays a key role in predicting the function of target proteins and genes. The main research methods of PPI are divided into three categories:in vivo, in vitro and in silico. This review compares the characteristics and limitations of these methods, summarizes the progress and application of protein-protein interaction technology in biology research, and prospects this technology.