Abstract: Effect of antimicrobial lipopeptides (AML) in fishmeal on the nutritional composition of the meat from Darkbarbel catfish (Pelteobagrus vachelli) was studied. Five diets incorporated with 0, 0.5, 1, 2, and 4% of AML were used to feed randomly selected 4 groups of fish comprising 20 individuals each for 8 weeks. The amino acids and fatty acids in the fish muscles were analyzed. The results showed no significant differences in protein or ash contents among the fish fed with the varied amounts of AML (P>0.05). On the other hand, the fish meat was considered of high-quality protein according to FAO/WHO standards. The greatest amount of amino acid in the meat was Glu, followed by Asp and Arg. The rich content of Lys was ideal to supplement grains for human nutritional need. AML did not show any significant effect on the amino acid contents (P>0.05). The fish meat contained more than 11 kinds of fatty acids, including 3 saturated, 4 monounsaturated, and 3 polyunsaturated fatty acids. There was no significant differences on the fatty acid contents found in meat from the fish fed on diets with 5 different AML levels either.