Abstract: The peduncle axillary buds of Zelglossoda Calico Gem, Green Valley #1, were used as explants for the study. The bud clumps induction pathway was experimented with orthogonal design to study the effects of the key factors, such as culture medium (i.e., MS, Hyponex #1, Improvement #1, Improvement #2, and Improvement #3), phytohormone (i.e., 6-BA, NAA, and IBA), and CH, on the plant growth during the induction, propagation, and rooting stages. The results showed that the main factors affecting the bud multiplication included 6-BA, basic medium, NAA, and CH. The optimal growth was observed under the use of the combination of Improvement #1 medium, 6-BA 3.0 mg·L^-1, NAA 0.1 mg·L^-1, CH 0.5 g·L^-1 and agar powder 5.0 g·L^-1 to result in an averaged propagation coefficient of 5.8 in 50 days. For the rooting, Improvement #3 with added IBA 0.5 mg·L^-1,AC 0.5 g·L^-1, sugar 20 g·L^-1,agar 3.6 g·L^-1 and carrageenan 3.6 g·L^-1 was the best with a 100.0% rooting rate. The survival rate of the plantlets in 6 months after transplanting from the test tubes was 96.8%.