高效液相色谱/四级杆飞行时间质谱法筛选产溶血磷脂酸芽胞杆菌

Screening Lysophosphatidic-acid-producing Bacillus Using HPLC and Quadrupole Time-of-flight Mass Spectrometry

摘要: 溶血磷脂酸（lysophosphatidic acid，LPA）是一种重要细胞外信号递质和细胞内第二信使。本研究采用高效液相色谱/四级杆飞行时间质谱（HPLC-QTOF-MS）技术对34株不同种类芽胞杆菌的代谢物进行测定。结果表明，供试的菌株中只有1株枯草芽胞杆菌Bacillus subtilis FJAT-8784的代谢物中检测出产溶血磷脂酸，与Metlin代谢物质谱数据库中溶血磷脂酸的匹配得分达96.73，该化合物的质子化分子离子M+H⁺质荷比为439.281 9，质量数为438.275 8，推断出其分子式可能为C₂₁H₄₃O₇P，其相对含量占发酵上清液总代谢物的1.32%。

Abstract: Lysophosphatidic acid (LPA) is an important extracellular signal transmitter and intracellular secondary messenger. Metabolites in the supernatants of liquid culture media for 34 strains of Bacillus were determined by using HPLC and quadrupole time-of-flight mass spectrometry. It was found that the only B. subtilis strain that produced LPA was FJAT-8784. The obtained LPA showed a matching score of 96.73 according to the Metlin database. It had a molecular weight of 438.2758, and a mass to charge ratio of 439.2819. Consequently, the chemical formula for the isolated LPA was assumed to be C₂₁H₄₃O₇P. In the supernatant, the total metabolites contained approximately 1.32% LPA.