猪巨细胞病毒福建株gB基因的克隆和序列分析

Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian

摘要: 为明确猪巨细胞病毒福建株（PCMV-FJ01）的gB基因特征，根据其序列特征，设计特异性引物对其进行分段扩增，并对目的片段进行克隆测序。结果发现，PCMV-FJ01株gB基因全长为2 580 bp，编码859个氨基酸；其和GenBank数据库中的PCMV分离株的gB基因的核苷酸和氨基酸同源性分别均在98.3%和97.9%以上。此外，本研究还发现部分（约50%）PCMV代表株的gB基因存在ACA三核苷酸缺失（436位氨基酸存在谷氨酰胺Q的缺失）。除ZZ株外，PCMV不同分离株的gB基因是否存在基因缺失和其遗传进化正相关。

Abstract: Based upon the characterization of gB gene retrieved from GenBank, specific primers were selected to amplify the gB gene of porcine cytomegalovirus (PCMV) FJ01 isolated in Fujian. The target PCR fragments were then cloned and sequenced. The gB gene was found to have 2 580 nucleotides, encoding 859 amino acids. Its homologies on nucleotide and amino acid sequences with the data at GenBank were greater than 98.3% and 97.9%, respectively. In addition, nearly half of the PCMV isolates had a same ACA nucleotide deletion in their gB genes that correlated to the phylogenetic evolution of the isolates, except ZZ strain.