Abstract: Male gamete development is an essential step in rice reproduction process, and male sterility is crucial for the utilization of rice heterosis. Since the anther or pollen specific genes in rice might play a pivotal role in the male gamete development, the functions of OsIPA were of great interest to the scientists. This study applied CRISPR/CAS9 to generate an OsIPA mutant for an investigation. Two sites in the 1^st exon of OsIPA were selected as the targets. After sequencing, a total of 8 different types of mutant, including deletion, substitution and single nucleotide insertion, were found in 24T₀ transgenic plants.