Abstract: In this paper, an experimental concentrated inactivated vaccine and a conventional inactivated vaccine against Novel Duckling Reovirus (NDRV) infection were prepared. After one single-dose immunization in adult Cherry Valley ducks, the serum samples collected in a period of 13 weeks were tested for antibodies by serum neutralisation (SN) method. The protective effects against NDRV infection were observed in 3-day-old Cherry Valley ducklings and mule duckling after a single-dose immunization. The serological results showed that concentrated inactivated vaccine was much more immunogenic than the conventional inactivated vaccine, with a much higher SN antibody titers at all test time points. The concentrated inactivated vaccine was highly protective against NDRV challenge at 7-day post-vaccination with a 100% protective efficacy, as compared to 40%-60% protective efficacy for conventional inactivated vaccine. Taken together, this experimental concentrated inactivated vaccine exhibited immune efficiency much superior to conventional inactivated vaccine and might be a useful vaccine against NDRV infection.