白茶实时荧光定量PCR分析中内参基因的筛选与验证

Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea

摘要: 利用GeNorm，NormFinder，BestKeeper软件对茶树不同叶位及白茶萎凋过程中7个候选内参基因的表达稳定性进行分析。获得3个稳定性较好的内参基因β-Actin、GAPDH和RUBP，RUBP适合作为白茶萎凋过程叶片荧光定量PCR的内参基因，β-Actin适合作为白茶不同叶位叶片荧光定量PCR的最佳内参基因。因此，在白茶萎凋过程中，使用GAPDH+RUBP组合作为内参基因进行荧光定量PCR检测。

Abstract: To select appropriate reference genes to be used in the real-time florescence quantitative PCR analysis for studying the gene expression in of white tea leaves during withering, the software of GeNorm, NormFinder, and BestKeeper were applied. Three stable reference genes, β-Actin, GAPDH and RUBP, were identified. Among them, RUBP was found most adequate for comparing the expressions at various stages of withering, while β-Actin for differentiating them in leaves from different parts of a tea plant. The combination of GAPDH+RUBP was determined as choice reference genes for the analysis.