Abstract: To understand the functions of eIF5A gene family in Phalaenopsis equestris, the genome database and bioinformatics were applied to obtain their gene structures, coded proteins, and predicted phosphorylation sites for analysis. The results showed that the eIF5A1 and eIF5A2 genes in P. equestris genome contained 5 and 6 extrons, respectively. The multiple alignments and motif displays rendered from the MEME method indicated that all PeeIF5A1 and PeeIF5A2 proteins contained one conserved DNA binding OB binding motif, PF01287. The phosphorylation site prediction on the proteins showed numerous sites.These results would provide the important clues for functional studies to reveal the role of PeeIF5A1 and PeeIF5A2 proteins in P.equestris.