Abstract: To establish an in vitro culture system for propagating Dan-Shui pear, Xiangshui pear was used on Murashige and Skoog medium (MS) as the basal medium for the experimentation. Conditions of the tissue culture, such as hormone combination and concentration on explant induction and proliferation culture, as well as hormone types and concentrations on rooting culture, were studied. The optimal combination for the axillary bud induction was found to be MS+ 6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1 that yielded a germination rate of 71.7%. For the bud proliferation, it was MS+6-BA 3.0 mg·L^-1+NAA 0.20 mg·L^-1 to deliver a multiplication coefficient of 3.2. For the optimal rooting performance, the combination of 1/2 MS+IBA 0.3 mg·L^-1 to result in a rooting rate of 63.3% was selected. Thus, addition of the growth regulators to MS medium benefitted the in vitro propagation of Dan-Shui pear.