Abstract: Auricularia polytricha is an economically important mushroom species in southern China. Nutritional compositions were compared between normal color and brown color variant groups. According to national standards, nutrition in brown species and normal species of Auricularia polytricha were compared. Results showed that the contents of protein, reducing sugar, vitamin B1 and vitamin B2 of Auricularia polytricha is significantly lower than brown species. The contents of E/P value and Fe of Auricularia polytricha is significantly higher than brown species. But results on fatty, crude ash, dietary fiber, energy maintained similar between them. Both of them contained more than 18 kinds of amino acid. The content of each amino acid, essential amino acids, no-essential amino acids, essential amino acids for child, all amino acids, sweet amino acids, acidic amino acids, bitter amino acids, flavor amino acids, sulfur amino acids, branched-chain amino acids and aromatic amino acids in brown species is higher than normal species, but E/T、E/N is lower than normal species. The nutritional value of AAS, CS and SRCAA in brown species is higher than normal species. It indicated that significant differences were observed on nutrition traits between these two groups and the nutrition of amino acids in brown species was better than normal species.