Abstract: The genetic relationship among 17 representative tea cultivars from various regions in central Yunnan was analyzed using ISSR markers.Out of the randomly selected 28 primers in testing DNA polymorphism of the teas, 15 produced distinctive bands and were chosen for amplification.The ISSR-PCR amplified primers showed 112 stable and clearly defined bands.Of which, 103 were polymorphic (a rate of 91.96%) averaging 7.4 bands each.The GS scores on the cultivars ranged from 0.58 to 0.80 with discrete classifications of Group Ⅰ that consisted of 12 germplasms and Group Ⅱ that composed of the remaining 5 germplasms.