Abstract: Using Fujian germplasm and Sichuan mutant of Auricularia polytricha as parents in a monospore cross breeding, the new strain named Za 10 with high yield, good quality and strong resistance was generated.The mycelia of Za 10 were dense, white in color, vigorous in growth, strong on resistance, and uniform in fruiting.The chrysanthemum-like fruiting body had apparent gill folds on bottom of pileus and fine fluff on cap.It was rich in pectin, and showed clearly separated black and white zones on dried product.In the regional trial, the dried mushroom yield was 66.22 g·bag^-1, that was 7.86% more than control.The protein, polysaccharide and amino acid contents in the fruiting body were 23.7%, 27.36%, and 15.55%, respectively, which were higher than those in control.The nutritional incompatibility and RAPD labeling tests showed distinct genetic differences between Za 10 and its parents.With the demonstrated advantages, Za 10 was recommended to be further tested for demonstration and promotion.