Abstract: Anthurium crystallinum seedlings were aseptically obtained from seeds for the study. Stem segments with node, leaves and petiole were used as plantlets, and the main factors of callus induction and differentiation as well asthe bud proliferation and seedling rooting during the propagating process were investigated. The results showed that theseed disinfection could be satisfactorily achieved by treating the peeledseeds with 0.1% mercuric chloride for 8min; the optimal callus differentiation of theplantlets was to use the stem segment with node, and cultivated in medium containing 1/3 MS+6-BA 1.0 mg·L^-1+2, 4-D 0.3 mg·L^-1; the appropriate medium forthe bud proliferation was MS+6-BA 2.0 mg·L^-1+NAA 0.3 mg·L^-1; that the medium for optimal rooting was MS+NAA 0.2 mg·L^-1; and a transplanting survival rate up to 95% could be achieved as a result.