Abstract: Pine wilt disease (PWD) is a devastating disease, which affects on the growth of masson pine (Pinus massoniana), often leading to withering and death. To reveal the change of gene expression pattern on this process, the gene dynamic expression from the P. massoniana infestation with nematode (Bursaphelenchus xylophilus) for 1, 2, and 3 days was studied. The genes, which were relevant to pathogen recognize, stress regulation, secondary metabolism, detoxification, and auxin-response were concerned. The results showed that most of the tested genes were presented highest expression level on the P. massoniana with infestation for two days, it was then down-regulated on these groups with 3-day infestation. Besides, the gene expression of CC-NBS-LRR resistance protein was continually increased in the treated P. massoniana compared with the corresponding control group. Additionally, genes expression of flavonoid 3-hydroxylase and cytochrome P450 were firstly up-regulated and then down-regulated on the P. massoniana respectively infected by graded increasing nematode qualities. The current findings preliminarily revealed the gene expression pattern on the P. massoniana in response to B. xylophilus infestation.