Abstract: The nutritional value of mushrooms grown on selenium (Se)-enriched medium was studied. Hypsizigus marmoreus No.10 was cultivated on JUNCAO No.24, and a local formula enriched with Se. The yield, conversion rate, fruiting traits.Se content and composition of fruiting body of the mushrooms were determined. The results showed that the amount and means of Se addition as well as the substrates in the cultivation affected the yield, conversion rate and properties of the mushrooms grown on the medium. In the concentration range tested, the Se content of H. marmoreus cultivated on the local formulation increased to 0.46-52.29 mg·kg^-1, 2.7-307.6 times of that of control; whereas, on JUNCAO No. 24, the mushrooms grew to contain Se 1.18-84.92 mg·kg^-1, which was 6.94-499.53 times of that of control.The Se content of the fruiting bodies of the mushrooms increased with the increasing Se of the medium. But the contents of water, ash and crude protein of the mushrooms inversely correlated with the Se concentration in the substrate. The Se appeared to promote the polysaccharide formation in the fruiting body, but showed no significant effect on either sugar or fat synthesis.