Optimization of Polysaccharide Extraction from Mycelia of Selenium-rich Agaricus blazei
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摘要: 为探讨姬松茸富硒菌丝体多糖的提取工艺，研发食用菌富硒菌丝多糖保健品，通过单因子试验和正交试验，对热水浸提法提取姬松茸富硒菌丝体多糖的条件进行优化。结果表明：料水比、乙醇沉淀浓度和浸提的温度、时间及次数对姬松茸富硒菌丝体多糖的提取均有较大影响，其最优提取条件为：料水比1：35（g·mL-1）、乙醇沉淀浓度90%、浸提温度85℃、浸提时间3.5 h、浸提次数3次，在此工艺条件下富硒菌丝体多糖的提取量及其硒含量分别达到69.5 mg·g-1和12.1 μg·g-1（菌丝干重）；本工艺简单、稳定、可行，适用于姬松茸富硒菌丝体多糖的提取。Abstract: The hot-water extraction of polysaccharides from the mycelia of Agaricus blazeia grown on a selenium-enriched medium was optimized to facilitate product development of health foods with the functional ingredient. A single factor followed by an orthogonal experiment was carried out to show the extraction yield to be significantly affected by the altered processing conditions. The optimized extraction conditions included a sample/water ratio of 1/35 (g·mL-1), an ethanol concentration for precipitation at 90%, a temperature of 85℃, and 3 repeated cycles that lasted 3.5 h. As a result, a yield on polysaccharides reached 69.5 mg·g-1; and the content of selenium in polysaccharides reached 12.1 μg·g-1(dry weight of mycelium). The procedures were simple, stable, practical, and appropriate for the extraction.
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Keywords:
- Agaricus blazei Murill /
- selenium-rich mycelium /
- polysaccharides /
- extraction
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图 1 料水比对多糖提取量的影响
Figure 1. Effect of sample/water ratios on extraction yield of polysaccharides
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图 2 浸提温度对多糖提取量的影响
Figure 2. Effect of temperature on extraction yield of polysaccharides
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图 4 浸提次数对多糖提取量的影响
Figure 4. Effect of repeated cycles on extraction yield of polysaccharides
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图 5 乙醇沉淀质量分数对多糖提取量的影响
Figure 5. Effect of ethanol concentration for precipitation on extraction yield of polysaccharides
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